|
Lysogeny broth (LB), a nutritionally rich medium, is primarily used for the growth of bacteria. The initialism is also commonly, albeit incorrectly, taken to mean Luria broth, Lennox broth, or Luria-Bertani medium. According to its creator Giuseppe Bertani, the abbreviation LB was actually intended to stand for lysogeny broth. The formula of the LB medium was published in 1951 in the first paper of Bertani on lysogeny. In this article he described the modified single-burst experiment and the isolation of the phages P1, P2, and P3. He had developed the LB medium to optimize ''Shigella'' growth and plaque formation.〔〔Bertani, G. (1951). Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J. Bacteriol. 62:293-300. PMID 14888646 (PDF )〕 LB media formulations have been an industry standard for the cultivation of ''Escherichia coli'' as far back as the 1950s.〔〔〔〔〔 These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. It continues to be one of the most common media used for maintaining and cultivating laboratory recombinant strains of ''Escherichia coli''.〔 For physiological studies however, the use of LB medium is to be discouraged.〔Nikaido, H. (2009). The Limitations of LB Medium. Small things considered - The Microbe Blog. ASM. http://schaechter.asmblog.org/schaechter/2009/11/the-limitations-of-lb-medium.html〕 There are several common formulations of LB. Although they are different, they generally share a somewhat similar composition of ingredients used to promote growth, including the following: * Peptides and casein peptones * Vitamins (including B vitamins) * Trace elements (e.g. nitrogen, sulfur, magnesium) * Minerals Peptides and peptones are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Tryptone is used to provide essential amino acids to the growing bacteria, while the yeast extract is used to provide a plethora of organic compounds helpful for bacterial growth. In his original 1951 paper, Bertani used 10 grams of NaCl and 1 gram of glucose per 1 L of solution; Luria in his "L broth" of 1957 copied Bertani's original recipe exactly.〔 Recipes published later have typically left out the glucose. == Formulae == The formulations generally differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions. The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics.〔 http://www.exptec.com/Reagents/Common%20Media.htm〕 * LB-Miller (10 g/L NaCl) * LB-Lennox (5 g/L NaCl) * LB-Luria (0.5 g/L NaCl) 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「lysogeny broth」の詳細全文を読む スポンサード リンク
|